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The LabChip90 analyzed 96 proteins at a time with analysis time of 40 seconds per sample.
The analysis time using MALDI-based assays is considerably shorter than using LC-based assays; in this case samples were measured at a speed of 13.5 seconds per sample (triplicate analysis), which is approximately 25 – 100 times faster than the LC-based approaches [2], [29] [31].
Table 2 gives the average computation time (in seconds) per sample.
A 96-well plate could be measured in approximately 48 minutes (30 seconds per sample) using 96 scans per sample.
The emission intensity was determined as relative light units (RLU) with a counting time of 5 seconds per sample.
Infected viable cell counts were measured by collection for a constant period of time (50 seconds) per sample.
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The whole process took less than five seconds (metabolites) or 10 seconds (transcripts) per sample from sampling to flash freezing in liquid nitrogen.
For audio preservation, it is important that the vendor at least has the capacity to produce 24 bit (per sample)/ 96 kHz (samples per second) uncompressed audio streams.
Data were collected on a Gold CCD camera in ten one-second exposures per sample.
Total RNA of brains and imaginal discs from 10 third instar larvae per sample was isolated using TRIzol Lifee Technologies, Carlsbad, California, USA) chloroform extraction and isopropanol precipitation.
The must obtained after crushing the first 20 berries per sample was immediately filtered through a strainer and a drop was used for °Brix analysis with a temperature-compensating CR50 refractometer (Maselli Misure Spa, PR, Italy).
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