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Cells were subjected to 3 cycles at the maximum agitation setting for 10 seconds with at least 10 seconds of cooling on ice between each agitation.
Samples were lysed for 30 minutes in lysis buffer on ice, and the chromatin was sheared by sonicating 20 times for 40 seconds each at maximum power with an ultrasonic processor, including 30 seconds of cooling on ice between each pulse (Misonix, Inc., Farmingdale, NY, USA).
DNA preparation for whole genome shotgun sequencing (WGSS) was done by shearing approximately 10 ug DNA for 10 min using Sonic Dismembrator 550 (cup horn, Fisher Scientific, Canada) with a power setting of "7" in pulses of 30 seconds interspersed with 30 seconds of cooling, and analyzed on a 8% PAGE gel.
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Diatom data suggest that during the first and second phases of cooling, the Sea of Azov was characterized by conditions of shallow waters with relatively high salinity and specific hydrodynamics.
One of Dr Weiss's former students, Sarah Parcak, of Cambridge University, presented data to the meeting on how a third period of cooling and drying, 4,200 years ago, destroyed the Old Kingdom of Egypt.Ms Parcak re-analysed a number of satellite photographs to produce a comprehensive survey of "tells" in part of the Nile delta.
Copper may be applied only in front of the first row of cooling passages.
All of them start from the refrigerated temperature (Tc) and there is a first stage of cooling until the initial freezing temperature (Tm) is reached.
The mean blood flow during two minutes preceding indirect cooling (LDPIh) was used as a reference against which the lowest value of perfusion during the first minute of cooling (LDPIc) was compared.
Contrasts were employed within Limma to detect probes for which the change in expression between the first day of cooling and the control was significantly different to the change in expression between the last time points and the control.
It was where pregnant women collapsed and tourists looked stricken, where people took Poland Spring showers and platform conductors jumped into air-conditioned cars solely for the 10 seconds of cool air they could steal before the train doors closed again.
The recent intense Leonid meteor storms have seen a rejuvenation of optical meteor studies, including the first application of cooled CCD technologies and the first large-scale use of intensified slit-less video spectrographs.
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