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Samples were microwaved (700 W) for 3×60 seconds with 30 seconds intervals between each, 1 ml of distilled water was then used to extract the trehalose for 1 hour.
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There was at least a 15 seconds interval between two trains of stimuli.
The homogenates were sonicated 4 times for 30 seconds, with 30 seconds interval between each sonication and then, centrifuged to separate supernatant and pellet.
Figure 4 (e) shows the relative vessel density (normalized to the baseline that was captured at the normal state) change during the progress with 30 seconds interval between the time points.
Real duty, he learned, is more like living inside a working clock — keeping watch by a relentless schedule, with sleep parcelled into shifts of a few hours and conversations carried on in fifteen-second intervals between foghorn blasts.
The right tibiae/fibulae in C57BL/6 mice were subjected to a single period of axial loading (40 cycles at 10 Hz with 10-second intervals between each cycle; approximately 7 min/day, 3 alternate days/week, 2 weeks).
The IAPS photographs were mounted on a stand and shown for 10 seconds with 10-second intervals between photographs.
The exercise protocol contains 5 sets * 10 repetitions of maximum contraction of elbow flexion at 50% MVC and 90 second intervals between sets.
Frozen liver or muscle tissues (50 100 mg) were homogenized in ice-cold de-ionized water (20 ml water/g tissue) and sonicated three times for 20 seconds with 30-second intervals between pulses, using a Misonix XL2020 ultrasonicator.
The sciatic nerve was subsequently stimulated with 9 mA/75 Hz bipolar pulses 5 times, for 0.1 second each (8 periods) with 5-second intervals between the pulses (Broniatowski et al. 1992, Silverman and Brull 1993).
However, over four minutes, unrelaxed force develops due to an extreme elongation of relaxation time so that the muscle does not relax to baseline during the two second intervals between stimulus trains (Fig. 2).
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