Sentence examples for seconds denaturation step from inspiring English sources

This included a 30 seconds denaturation step at 95°C, a 30 seconds annealing step at 55°C (Figα) or 57°C (PAR6 and β-actin), and a 40 seconds extension step at 72°C.

After an activation cycle with 90°C for 10 Minutes, 45 cycles were performed with a 10 seconds denaturation step at 95°C, 30 seconds of annealing at 60°C and polymerase extension for 1 second at 72°C.

After 6-minute initial denaturation at 96°C, a "hot start" amplification was initiated by adding DreamTaq DNA polymerase, followed by 19 cycles consisting of a 15 seconds denaturation step at 95°C, a 30 seconds annealing step at 64°C, and a 15 seconds extension step at 72°C, with a final extension for 45 minutes at 72°C.

The thermal cycler was programmed for a first denaturation step of 5 min at 95 °C, followed by 35 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s.

PCR was performed with Phusion DNA polymerase (Thermo Scientific) under the following cycling parameters: 30 cycles of 98 °C for 10 s, 59 °C for 1 min and 72 °C for 1 min, with a first denaturation step at 98 °C for 30 s and a final extension step at 72 °C for 10 min.

The HRM step consisted of a first denaturation step at 95°C [1 min], followed by a renaturation step at 40°C [1 min].

Thermal cycling was initiated with a first denaturation step at 95°C for 2 minutes, followed by 45 cycles of 95°C for 15 seconds and 60°C for 1 minute.

Thermal cycling was initiated with a first denaturation step at 95°C for 3 minutes, followed by 40 cycles of 95°C for 15 seconds, 58°C for 30 seconds, 72°C for 30 seconds.

Thermal cycling was initiated with a 2-minute incubation at 50°C, followed by a first denaturation step of 10 minutes at 95°C, and then 40 cycles of 95°C for 15 seconds and 60°C for 1 minute.

Thermal cycling was initiated with 2 min incubation at 50°C, followed by a first denaturation step of 10 min at 95°C, and then 40 cycles of 15 sec at 95°C and 1 min at 60°C.

After the initial 30 second denaturation step, we performed multiple cycles each consisted of 10 seconds denaturation at 98°C, 30 seconds annealing at 55°C, and extension at 72°C for 20 30 seconds per kb according to the length of the longest piece.