Sentence examples for seconds cycle from inspiring English sources

Peptides were eluted directly into the electrospray source of the mass spectrometer (6490 Triple Quadrupole, Agilent) operating in scheduled MRM positive-ion mode (Q1 resolution: wide; Q3 resolution: unit; detection window variable: 124 to 240 seconds; cycle time: 1.0 seconds).

The cells were then homogenized by ultrasonication for 40 seconds (cycle 1, amplitude 70%) in a sonicator (UP 100 H, Dr. Hielscher GmbH), and the total protein content was measured using a modified method originally developed by Lowry [ 18, 27].

The PCR amplification was performed in a 7900HT Fast Sequence Detector System Applied Biosystemss) with the following program: Cycle 1, 95°C for 10 minutes; Cycle 2, 40 cycles of 95°C for 15 seconds, 60°C for 60 seconds; Cycle 3, 95°C for 15 seconds, 60°C for 15 seconds, 95°C for 15 seconds.

Thermal cycler conditions were: initial denaturation at 94° for 3 minutes, followed by 10 cycles of (94°C for 20 seconds, 50°C for 20 seconds, 72°C for 3 minutes 30 seconds), cycles 11 – 30 added 10 seconds/cycle to the 72°C extension, and ended at a 15°C hold.

"Took only about two days to get right back to my check-it-every-five-seconds cycle".

"Took only about two days to get right back to my check-it-every-five-seconds cycle". He went from reading something like a book a week to reading a book a month: a projected total loss of 36 books per year.

(Wolfgang Neumann is Siegfried in the second cycle).

And now it's in its second cycle and then it's -- in its third cycle.

The discharge overpotential needed for the second cycle was 200 250 mV larger than the first cycle.

Rough diamond sales for the second sales cycle rose to $610m from $545m in the first cycle.

Best choice of tickets is for the third cycle.