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Sonication was performed for 8 minutes, with 20 second pulses followed by 40 seconds "cooling off" period.
This was performed with a Qiagen Tissue Lyser: one minute at speed 30/second followed by 30 seconds cooling the tubes on ice, repeated three times.
First, cool the air to well below the desired room temperature.
To the degree that America First aligns with Back People First, cool.
Here, hot desert air going into a greenhouse is first cooled and then humidified by seawater.
To decorate the cake, spoon some buttercream on top of the first cooled sponge and spread evenly.
The samples were first cooled to the LN2 temperature and then gradually heated to room temperature.
Second, cooling of SWNT (at TCNT ) within water (at Tw) was accomplished by NVE simulations.
To induce phase separation, the polymer solution is first cooled rapidly.
The simulation showed that the extra shade first cools the covered area, but that temperature decrease changes local weather patterns.
Gelation was reversible upon heating, although after a second cooling cycle the HC-MCC gel had lower G′.
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CEO of Professional Science Editing for Scientists @ prosciediting.com