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Cells were resuspended in 80 µl of the fibrinogen solution and, a few seconds before injection in the tissue, 20 µl of thrombin (250 UI/ml) were added to the syringe containing the cell suspension.
Ten seconds before injection of radioactive water, the stimulus was started.
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No PSA-specific T-cells were detected when electroporation was applied seconds before DNA injection (supporting information Fig. S1).
For dynamic PET analysis, scans were started 5 seconds before radiotracer injection and continued for 90 minutes.
Hold your breath a few seconds before the injection and while it is being administered.
Tumor growth and mouse weight were assessed three times a week in the first week before injection and the 3 weeks after injection; thereafter, weight, growth, and survival were assessed twice a week.
Of the 134 infants who cried, the median total cry time was 33 seconds (IQR = 39), with a maximum of 146 seconds.Infants' pain during the immunizations was coded using the FLACC scores at four different time points: baseline (15 seconds before the first injection), at the time of the first injection, at the time of the last injection, and 15 seconds following the last injection.
X-ray image acquisition was initiated from one second before iodine injection, and continued for five seconds.
Even though the gene profiling data support the hypothesis that electroporation has an adjuvant effect, no induction of PSA-specific T cells was detected when electroporation was applied seconds before the DNA injection.
In the L-arginine experiments L-NAME (1.2 mmol/kg) or saline (controls) was injected i.v. 10 seconds before the i.v. injection of L-arginine (3.6 mmol/kg).
The first frame was acquired before injection of a paramagnetic contrast agent (gadopentetate dimeglumine, 0.1 mmol/kg body weight, Magnevist™, Schering, Berlin, Germany), which was immediately followed by five other measurements.
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