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The microplate was then thermocycled as follows: initial denaturation of 94°C for 2 minutes, then 55 cycles of denaturation at 94°C for 5 seconds, annealing at 52°C for 5 seconds, and extension at 72°C for 5 seconds before cooling to 4°C.
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Coming off a 35-point performance last Sunday at Canisius, Flores, a junior guard, scored 17 first-half points before cooling off in the second half.
The premier line on the Islanders' playoff team two seasons ago, the three combined for 18 goals in the first 13 games this season before cooling off.
However, this configuration benefits from the lower temperature of the cooling air, which has not to pass through the cooling package before cooling the ORC radiator, and can, in first approximation, be considered at the same temperature of ambient air.
before cooling down to 4°C.
Let the cooling system focus on that room first, before the cooling must occur in any other part of the house.
After removing the cookie, give it a couple (and only a couple) seconds to cool before putting the fortune in.
Camby's premeditation -- he had 20 to 30 seconds to cool down before he rushed Ferry -- means more than a one-game suspension, the minimum for throwing a punch.
Blanch the nettles in salty water for seconds then cool them fast before squeezing them very dry.
Second, controlled surface cooling devices were not available before 2009 in our hospital.
Mabbott's are perfectly cooked, although I slightly prefer those from the blog Taste Hong Kong, which takes a similar approach but scalds them in boiling water for a mere 30 seconds before leaving them to cool and dry for half an hour.
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