Sentence examples for seconds at maximum from inspiring English sources

The extraction were carried out by sonication (3 cycles of 30 seconds at maximum amplitude on ice) in buffer extraction (NaCl 0,3 M, Tris-HCl 0,4 M pH = 8, 0.1% Tween 20).

We derive the energy (E) from the period (P, in seconds) at maximum amplitude using (ReVelle 1997): begin{aligned} text{log}_{10}left(frac{E}{2}right) = 3.34 text{log}_{10}(P) - 2.58 qquad E/2 le 100, text{kt} end{aligned} (1)In the period range and distance range in Table 2, the effect of atmospheric absorption is neglected and the peak period becomes independent from distance.

These mixtures were shaken for 30 seconds at maximum speed using a minibeadbeater.

The cross-linking was quenched with 2.5 ml of 2.5 M glycine, cells were collected and crushed by glass beads in LB/IP buffer (50 mM Tris.HCl, 140 mM NaCl2, 5 mM EDTA, 1x PI cocktail (Sigma)) and sonicated by 12 bursts of 30 seconds at maximum output of Micronix cuphorn to produce fragments of about 500 bp.

Centrifuge the remaining 900 μl for 15 seconds at maximum speed and resuspend in 100ul SOC.

After about 3 to 5 minutes of warm-up the subject cycles for 30 seconds at maximum effort against a standardized resistance.

The acquisition time for near-infrared fluorescence images was 1 second at maximum photon flux of the system.

Samples were lysed for 30 minutes in lysis buffer on ice, and the chromatin was sheared by sonicating 20 times for 40 seconds each at maximum power with an ultrasonic processor, including 30 seconds of cooling on ice between each pulse (Misonix, Inc., Farmingdale, NY, USA).

Photos can be sent back and forth, or to other people, within 20 seconds at a maximum resolution of 640 x 480 pixels.

The turret could be rotated 360 degrees in 60 seconds in low gear independent of engine rpm, in 19 seconds in high gear at idle engine speed, and within 10 seconds at the maximum allowable engine speed.

Post-activation images were recorded for 5 seconds at the maximum rate.