Sentence examples for seconds and cooled from inspiring English sources

The slides were then washed with TE buffer for 30 seconds and cooled to 45°C, washed with 0.5× TE buffer for 30 seconds and cooled to 40°C, washed with 5% ethanol (Sigma Aldrich, St . Louis MO) for 1 minute and cooled to 30 °C, and finally washed twice with ddH2O for 40 seconds and cooled to 25 °C.

The sample was mechanically lysed for 30 seconds and cooled on ice for 30 seconds, repeated eight times.

The reaction was heated to 95°C for 10 seconds, and cooled to 63 68°C for 5 20 minutes, during which time OCP annealed to genomic target and was circularized by ligase.

Blanch them in hot water with salt for 30 seconds and cool in ice water.

The PCR profile consisted of 10 minutes at 95°C, followed by 40 cycles with heating of 95°C for 15 seconds and cooling to 60°C for 1 minute.

Melting curve analysis was performed for 1 cycle with a ramp rate of 0.2°C per second, starting at 94°C for 5 seconds, 72°C for 30 seconds, and back to 94°C for 0 seconds, and cooling at 40°C.

Amplification conditions were: 95°C for 2 minutes, 45 cycles of 94°C for 30 seconds and 55°C for 30 seconds, followed by 1 cycle at 94°C for 30 seconds and cooling to 25°C for heteroduplex formation.

By use of a peltier thermal element, the immobilised products kept in suspension were heated in deionised water to 80°C (1°C / 2 s) for 1 second and cooled to room temperature (1°C / 2 s).

At this time, the USSR achieved rough nuclear parity with the United States; meanwhile, the Vietnam War both weakened America's influence in the Third World and cooled relations with Western Europe.

Template DNA was denatured with reaction buffer, MgCl2, NP-40 and ddH2O for 4 min (first cycle), and cooled on ice immediately.

Instead of simply performing the usual ninth-inning job of an elite closer, he is being asked to rescue the Dodgers an inning earlier, getting the final out of the eighth and cooling down in the dugout before completing the ninth.