Sentence examples for seconds additional from inspiring English sources

TI duration is the time during which a bird remains immobile after the fear reaction has been induced by keeping it on its back in a U-shaped cradle for 10 seconds (Additional file 5: Figure S3).

General amplification protocol (40 cycles): initial denaturation for two minutes at 95°C; denaturation for 15 seconds at 95°C, specific primer annealing temperature (see above) for 15 seconds, amplification at 68°C for 20 seconds, additional heating step to 5°C below the melting temperature of the PCR product (see above).

Next, PAGE-purified primers were pooled together, and multiplex single-base extension reactions were performed on ExoSAP-IT-treated (USB/Affymetrix, Santa Clara, CA, USA) PCR products using the following conditions: 96°C (30 seconds), followed by 35 cycles at (96°C (10 seconds), 50°C (5 seconds) and 60°C (30 seconds)) (Additional file 3: Table S3).

General amplification protocol (40 cycles): initial denaturation for two minutes at 95°C; denaturation for 15 seconds at 95°C, specific primer annealing temperature (see table) for 15 seconds, amplification at 68°C for 20 seconds, additional heating step to 5°C below the melting temperature of the PCR product (see table).

In addition to ln(CRP), BMI (kg/m) was included in a second additional model.

Under the current scheme you can build up contributions to the basic and second additional pension.

Savers are currently able to pay into the second additional pension over their whole working lifetime.

Cases that follow a pattern would be the first additional John Doe indictments made, he said.

General studies and critical thinking, if they are one of three A-levels, rather than a fourth, additional A-level.

The second additional feature is the pattern of the absorption peaks.

President Obama sent the first additional 17,000 troops before he'd been in office a month and explicitly before coming up with any plan for Afghanistan.