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Secondly, read numbers of endo-sRNAs do not reflect steady-state mRNA levels of their parent genes.
Secondly, read 1 should map to the positive strand of C2T reference genome, or negative strand of G2A reference genome, while read 2 should be the exact opposite to read 1.
Secondly, read pairs where one mate is aligned to the genome reference uniquely (i.e. with high read mapping quality value, or MQ) and the other mate is either soft-clipped or unaligned, are collected as the starting material for SR mapping.
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Secondly reads were counted per exon with a python script (Lodder R. unpublished).
Secondly, I read Then and Now 10 years ago and haven't looked at it since.
The problem is that we all feel very warm and fuzzy about vitamins because, firstly, the tales of deficiency are so horrific, secondly, we read breakfast cereal packs and thirdly, a double-Nobel laureate called Linus Pauling liked vitamin C in vast, vast doses.
Secondly, I read the father's statement about his son being punished too harshly and this being only "20 minutes of his life".
Re-analysis with presence of pneumonia according to the (secondly) centrally read radiographs as the reference test showed a net reclassification improvement of 28%19%to37% 37%).
Secondly, the read coverage is high: for 200 kb upstream and downstream of aat1 on chr11, only 0.61% or 0.73% of bases showed a read coverage of < 5 or < 10, respectively (Additional File 5).
Secondly, combining read counts from multiple data sets where some binding occurs at loci common across many data sets and some binding occurs at loci specific to particular data sets will introduce unusual patterns variation in reads counts that could bias estimation of background rates.
Firstly, because its author is grossly under-appreciated and secondly, because reading him will give one fresh insights into recent short story greats such as George Saunders and David Foster Wallace.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com