Sentence examples for secondary were used from inspiring English sources

For Ki-67 staining, a primary rabbit antibody (clone H-300) and a biotinylated anti-rabbit secondary were used.

Rabbit anti-K5 or K6 antibodies (primary) and biotinylated anti-rabbit IgG (H + L) (Vector Laboratories, Cat: BA-1000) (secondary) were used, and signal detection was performed using the VECTASTAIN elite ABC Kit (Vector, Cat: PK-6100) and AEC REDD) single solution (Zymed, Cat: 00-1111) according to instructions from manufacturers.

For FACS analysis of H9 cells, anti-IgM-APC secondary was used to detect GCTM2.

The AlexaFlur 594-conjugated secondary was used followed by three washes with 1 × PBS/0.5%Triton X-100.

Alkaline phosphatase conjugated secondary antibodies were used at 1 10000 (Promega), and fluorescently labeled secondaries were used at 1 5000 (LiCor).

Anti-mouse HRP-conjugated secondary antibodies were used at 1 5000 for GluRIIA blots and anti-rabbit AP-conjugated secondaries were used at 1 1000 for GluRIIB and Sec8 blots.

"Primarily in Europe," said Nelis, "secondaries are used to providing liquidity to founders.

Both primary and secondary data were used.

For the surface analysis secondary electrons were used.

For the analysis, primary and secondary standards were used.

Both primary and secondary data were used for this study.