Sentence examples for secondary was applied from inspiring English sources
After washing with TBS, the Biogenex Polymer HRP Secondary was applied and sections were incubated for 30 min at room temperature.
The word secondary is applied to esophageal disorders in systemic disorders such as connective tissue disease, diabetes, dermatomyositis, amyloidosis, and Chagas' disease.
The anti-rabbit horseradish peroxidase secondary antibody was applied for 12 min at room temperature.
The coverslips were washed three times with phosphate-buffered saline (PBS), and secondary antibody was applied for 1 h at room temperature.
After washing, goat anti-rabbit AlexaFluor 488 secondary antibody was applied for 1 h.
After washing, HRP-conjugated secondary antibody was applied at 1∶2000 for 1 hour at room temperature.
After washing, the secondary antibody was applied and incubated for 30 min at room temperature.
Horseradish peroxidase-conjugated secondary antibody was applied to locate the primary antibody.
The secondary antibody was applied for two hours in 1.5% goat serum in PBS.
A Phycoerythrin-conjugated secondary antibody was applied for 30 min.
Appropriate secondary antibody was applied at a 1 250 dilution.
