Sentence examples for secondary was added from inspiring English sources

After washing in PBS, the biotinylated rabbit anti-hamster secondary was added (1 : 500 AbCam2 AbCam) for 1 h.

Then secondary antibody was reconstituted with the secondary antibody solution; 100  μl of constituted secondary antibody was added to each well.

In this method, PVX-specific antibodies were used as a capture, and accordingly, goat polyclonal secondary antibody was added.

But, after the unveiling there were so many inquiries about them that a secondary label was added".

Cy3 conjugated secondary antibody was added (Jackson Immunoresearch Laboratories).

Secondary antibody was added and incubated for 1 hour at room temperature.

After thorough washing with PBS, the secondary antibody was added for 3 h at room temperature.

After washing, an Alexa Fluor donkey anti-goat secondary antibody was added for 1 h.

Cells were then washed 3× in PBS, and secondary antibody was added in PBS with 10% FBS.

The cells were washed three times with PBS-BSA, and phycoerythrin-conjugated secondary antibody was added for 1 h.

After the plate was washed, horseradish peroxidase conjugated secondary antibody was added for 1 h at room temperature.