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A secondary validation was done at the thick layer level.
Third, the high accuracy achieved by cross-platform validation obviates the need for secondary validation (Fig. 3, Supplementary Note 7).
As a secondary validation of these predictions, the European Medicines Agency EurdaVigilance database of spontaneous ADRs was queried for reports of the same association (Table 5).
However, clinical adoption of WGS has been limited to pilot studies involving very small numbers of cases (mostly < 10 12,13,14,15,16,17 and the median turnaround time for generating the final clinical report may exceed 100 days due to mandatory secondary validation of variants by a CLIA-certified lab12,18.
As treatment of pediatric cancer typically costs hundreds of thousands of dollars, the precision of integrative genomic profiling offered by three-platform sequencing outweighs the extended time for secondary validation and the potential for missing ~ 20% pathogenic variants in pediatric cancer.
Secondary validation is required for any screening endeavor.
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Indeed, even if an antibody has satisfied other secondary validations, it is still good practice to perform a ChIP with two different antibodies, when available.
Both actin and DNA staining allowed a secondary visual validation of pre-mitotic cell rounding.
A secondary functional validation screening revealed that the potential oncogenes participate in different cellular processes affecting proliferation, cell viability, apoptosis or differentiation.
These candidates were subjected to secondary genotyping validation (see Methods S1).
Further many NetPath molecules and their reactions are supported by either single publications or derived from high-throughput experiments lacking secondary experimental validation.
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