However, homology is ambiguous in the N domain, particularly the extreme N terminus, where secondary structure is unconserved.
The addition of a deliberate secondary mismatch near the 3' terminus of an NLS primer has been demonstrated to improve allele-specificity by destabilising the binding of the primer and improving primer extension stringency [ 5].
The N- and C-termini and secondary structures are labeled.
Secondary structure elements and termini are labeled.
Further deletion constructs indicated that the primary oligomerization sites lie within the last two transmembrane regions near to the C-terminus, whereas secondary elements are also present within the preceding four transmembrane segments [ 6, 7].
CipA also interacts via its C-terminus with secondary scaffoldins, which anchor CipA and its associated cellulolytic enzymes to the cell wall and lead to the formation of more complex polycellulosome structures [ 10].
(A ) Ribbon diagram of zSmo CRD in rainbow coloring from blue (N-terminus) to red (C-terminus) with the secondary structure elements numbered.
Barry got into a grammar school but "halfwit" Richard was shunted towards the terminus of a secondary modern.
The secondary amine at its N terminus is normally not well recognized by enzymes of the M1 family of metallopeptidases with the GXMEN motif, which is responsible for interactions with the free amine in α position (Tholander et al. 2008).
We selected DISC1 amino acids 326 597 for analysis, fused to maltose-binding protein (MBP) tags, based upon considerations of domain delineation, predicted protein disorder in the N-terminus of native DISC1 and likely consequences for protein expression, purification, and finally on the assessment of secondary structure for the C-terminus of native DISC1 (15).
