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One of the correctly targeted clones, 1D4, was used in the secondary targeting with a 3′-Hprt vector construct.
Subsequent confocal microscopy and flow cytometry studies delineated how secondary targeting mechanisms affected the EGFR targeting.
Further evidence on the influence of secondary targeting was obtained using flow cytometry.
This secondary targeting was further confirmed by the diminished signal in the presence of excess HDL (Fig. 5b).
However, the contribution of the targeting ligand to nanoparticle delivery is often influenced by non-specific nanoparticle uptake or secondary targeting mechanisms.
Therefore, the secondary targeting of SR-BI along with the targeting to EGFR was investigated using confocal microscopy and flow cytometry.
Another interesting effect of this secondary targeting lies in the fact both EGFR and SR-BI contributed to the EGF-HPPS uptake.
Thus, plastid proteins are first targeted to the outer membrane via a hydrophobic signal sequence and secondary targeting signals mediate uptake into plastids [ 47].
the L button for targeting; and the R button for secondary targeting.
The results will help define the primary and secondary targets and what about the movie most appeals to them.
A mechanical failure reduced the fuel supply, and both the primary target, the city of Kokura, and the secondary target, Nagasaki, were obscured from the air.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com