Sentence examples for secondary stains from inspiring English sources

Secondary stains were performed using avidin-biotin visualization systems (Vectastain ABC Kit, Vector Laboratories).

After 30 min of secondary stain with Alexa 488 anti-mouse IgG secondary antibody (for SSEA-4 stained cells), and PE-anti-mouse IgM antibody (for Tra-160 and Tra-1-80), cells were analyzed on BD LSR II flow cytometer.

Smooth muscle cells were identified using monoclonal mouse anti-human smooth muscle actin (1 50; Dako Inc .. Secondary staining was performed with HRP-conjugated respective secondary antibody, followed by diaminobenzidine (DAB substrate kit for peroxidase; Vector Laboratories, Burlingame, CA, USA), and counterstaining with Gill's hematoxylin solution.

The secondary staining was performer with the PE-conjugated rat anti-mouse immunoglobulin (BD Biosciences).

Allophycocyanin (APC) conjugated Streptavidin (BD cat 554067) was used for secondary staining of lineage markers.

4LO3311 supernatant was used and secondary stained using an anti-mouse IgG3 PE-Cy7 (Southern Biotech).

Both primary and secondary staining was conducted for 30 minutes at room temperature in FACS buffer.

Followed by secondary staining with HRP-conjugated goanti-humanmantibodyody (1∶2,000).

The majority of the FITC secondary staining was in the nucleus, thus this conjugate was not used for AKAP95 visualization.

Texas Red-tagged anti-rabbit IgG and FITC-tagged anti-mouse (or goat) IgG were for secondary staining.

Alexa Fluor® 568 fragmentfragment of goat anti-mouse IgG (Molecular Probes), Alexa Fluor® 488 fragmentfragment of goat anti-rabbit IgG (Molecular Probes) were used for secondary staining.