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Hartmann sees quantities as determinations of real being and qualities as (within limits) determinations of the secondary sphere of knowledge (A.39a).
As demonstrated here for Cyt b5, it may be of practical importance for design of artificial heme proteins by engineering a Tyr in the secondary sphere with improved properties and functions.
The active site of metalloproteases is composed of a primary sphere of residues co-ordinating the zinc atom, and a secondary sphere of residues that determines proteolytic specificity and activity.
These observations suggest that the conserved Tyr30 in Cyt b5 is not replaceable due to the presence of both the H-bond network and hydrophobic interaction in the secondary sphere of the heme active site.
To probe the stabilization role of conserved tyrosine residue in the secondary sphere of heme site in heme proteins, we herein used cytochrome b5 (Cyt b5) as a model protein, and mutated Tyr30 to Phe or His by removal of Tyr30 associated H-bond network and hydrophobic interaction.
However, while primary sphere formation was reproducibly increased in a dose dependent manner in established cell lines, secondary sphere formation was not increased and neither sphere formation nor CD44+CD24− cells were increased in MDA-IBC-3 tumors cultured with MSC.
Similar(33)
The main purpose of this book is to demonstrate that modalities ground the differences between the two principal spheres of being (real and ideal) and the two secondary spheres of being (knowledge and logic).
In more detailed models the seven planetary spheres contained other secondary spheres within them.
We performed the same assay for the differentiation of tertiary spheres that had been generated from secondary sphere-like colonies.
Immunocytochemistry revealed that 75% of the secondary sphere-like colonies generated neurons (β-III-tubulin), astrocytes (GFAP) and oligodendrocytes (CNPase; Fig. 5c).
We were unable to generate secondary spheres from the initial spheres, as has been shown for other tissues.
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CEO of Professional Science Editing for Scientists @ prosciediting.com