Sentence examples for secondary restriction from inspiring English sources

V. cholerae isolates were subtyped according to the PulseNet standardized pulsed-field gel electrophoresis (PFGE) protocol, using primary and secondary restriction enzymes SfiI and NotI, respectively (12 ).

NlaIII (New England Biolabs, Ipswich, MA) was used as the primary restriction enzyme and DpnII (New England Biolabs) was used as the secondary restriction enzyme.

The 3C-Seq libraries were prepared step by step as described by Stadhouders R et al.. HindIII and DpnII were used as the primary and secondary restriction endonuclease.

The presence of end range pain in internal rotation and flexion with a secondary restriction of abduction is a commonly reported capsular pattern of restriction for the hip joint [ 26].

The advantage of the secondary restriction enzyme over mechanically shearing is that it is very reproducible and provides a better repair step of the ends and hence ligation of the adapters.

Mechanically shearing would have the advantage that the chance of capturing a fragment is improved, because some of the secondary restriction sites are too close to the primary restriction sites.

Two groups can be distinguished: a group corresponding to fragments that do not exhibit a secondary enzyme restriction site (having a number of reads inferior to 1000) and a second group corresponding to fragments having a RE2 site.

American sanctions — including, in the case of Suleimani, far-reaching "secondary," extraterritorial restrictions — are more powerful than those imposed by the EU, or any other individual country, in part due to the US's outsized role in the world economy.

As the disease progresses, the respiratory system is further compromised by pulmonary restriction secondary to the effects of the disease on the thoracic skeleton.

Subjects also received fetal growth ultrasound scans at 28, 34 and 37 weeks of gestation to screen for intra-uterine growth restriction secondary to pre-eclampsia.

Fetal growth restriction secondary to known imprinted gene disorders are however rare and do not explain the majority of FGR seen in the developed world.