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This addressed a gray area in Japanese law which, in principle, renders a "secondary recipient" of inside information immune from punishment, a loophole that had been viewed as one obstacle to catching overseas funds involved in insider trading cases.
'Leukaemic' sample material was GFP-positive splenocytes collected from secondary recipient mice upon frank leukaemia development.
Cells recovered from one single primary host were transferred into one secondary recipient mouse.
Each secondary recipient mouse was reconstituted with a fraction of enriched cells equivalent to those from 1 femur.
The unsorted, CD44+ and CD44− cells were injected into secondary recipient nude mice and monitored for tumor formation.
For secondary transplantation, CD45.1+ cells were collected from primary recipients and 106 cells were injected with 105 competitorsetintos into lethally irradiated secondary recipient mice.
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The documents I have are silent on the issue of what happened to the eggs that were harvested and not transferred at the time of the harvest, so it is unclear whether any of the eggs I donated may have been frozen and used later, or if unused eggs or embryos could have been frozen and then passed on to secondary recipients.
We showed polyclonal engraftment, multi-lineage differentiation, and propagation to secondary recipients of individual SRC.
Furthermore, CD4+ T cells harvested from recipients of hFVIII expression vector-modified tDCs were able to mediate antigen-specific immune suppression in naive secondary recipients.
Three clones from E900 remained detectable in each of two secondary recipients, one of which was treated with, and responded to, AP1903.
The treatment did not affect the engraftment level or the SRC frequency, but seemed to enhance SRC susceptibility to LV. SRC transduced in both conditions repopulated primary and secondary recipients, maintaining stable multi-lineage transgene expression.
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