Sentence examples for secondary purification from inspiring English sources

The secondary purification step utilizes a gel permeation chromatography column, producing homogeneous INGAPrP as well as refolding the protein.

Features of the PowerClean Pro DNA and RNA Clean-Up Kits include: efficient secondary purification, removal of challenging impurities, and successful amplification.

Size-based secondary purification was then achieved by continuous-elution electrophoresis (Model 491 Prep Cell, Bio-Rad).

The RNA protein complexes were subsequently treated with TEV protease, followed by immobilized metal-ion affinity chromatography (IMAC) as a secondary purification.

In the iCLAP technique, Jernej Ule and colleagues, who invented the iCLIP method, used magnetic streptavidin beads in the first purification step followed by cobalt beads under denaturing conditions as a secondary purification step [26].

Assessment of treatment performance in the large-scale constructed wetland (CW) for secondary effluent purification remains limited.

This means that the AuNPs did not turn into the secondary pollution during water purification.

However, Pd diffuser is suitable for the secondary process, like the purification process, after the tritium in the sweep gas has been concentrated by another method.

A complicating factor is that B. orellana is recalcitrant to molecular biology studies, probably because its tissues contain high amounts of secondary metabolites that hinder purification of nucleic acids [ 16].

One of the vectors, pSANG14-3F, which produces scFv-fused to alkaline phosphatase, offers a simple and convenient way of determining antibody fragment binding in a variety of assay systems without the need for antibody fragment purification and secondary antibody reagent addition.

After cooling to room temperature, 10 μL 3M NaOAc pH 5.5 was added to each 100 μL secondary PCR reaction followed by purification with the QiaQuick PCR purification kit (Qiagen) using the manufacturer's recommended protocol.