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9. Wildchild: Secondary Protocol (Stones Throw).
Stones Throw continues to reign supreme with dope new releases like Wildchild's Secondary Protocol, Jaylib's "The Official" 12", J.Rocc's "Play This (One)" 12", and the supergood Shades of Blue: Madlib Invades Blue Note.
Around this time, Daniel realizes the Secondary Protocol, detailing the whereabouts of Ben Linus, has been activated by Widmore, necessitating their immediate departure from the island.
Upon returning to the freighter, Keamy unsuccessfully attempts to kill Michael Dawson Harold Perrineauu), whom he has discovered is Ben's spy, then obtains the "secondary protocol" from a safe.
An upgrade occurred if a secondary protocol outcome was promoted to a review primary outcome and a downgrade occurred if a protocol primary outcome was demoted to a review secondary outcome.
The universal secondary protocol and the DAB MAP kit (Ventana Medical Systems, Inc., Tucson, AZ, USA) were used to detect and amplify the signal.
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Sections were then washed in PBS and incubated with secondary protocols from ABC Vectastain kit (PK-4001; Vector Labs, Burlington, ON, Canada) and then visualized using diamino-benzidine (DAB) (Sigma) substrate.
A secondary per protocol analysis was conducted on all those patients in whom the treatment protocol was adhered to.
A secondary, per protocol analysis will be performed for the primary outcome, excluding patients with major protocol violations.
A secondary per protocol analysis will also be performed.
If indicated, a secondary, per protocol analysis will be performed.
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