No specific staining was observed with the rabbit and goat non-immune isotype IgG controls using the same conditions and secondary antibodies (negative control; Fig. 4E).
We cultured Rae-1 overexpressing Rae-1 overexpressingin a 12-well plate, stained the cells with the 52A anti–Rae-1 mAb and then with GFP-conjugated anti-mouse secoverslipstinody or secondary antibody alone (negative control).
Complete absence of immunoreactivity in the intertubular space and on the spermatozoa was noted in the epididymal tissue sections immunoreacted with normal goat serum or secondary antibody alone (negative control) (Fig. 5Da'-h').
Blank (no serum, secondary antibody only), negative control (anti-HMGCR negative serum) and positive controls (highly positive human anti-HMGCR Ab serum or rabbit anti-HMGCR Abs with appropriate secondary antibody) were included in every assay.
Cells incubated only with the secondary Ab served as negative control for non-specific antibody binding.
No unspecific binding of the fluorochrome-conjugated secondary antibody to neurons (negative control) was found.
Immunolabelling of Arabidopsis cryo-fixed thin root tissue sections with rabbit pre-immmune serum and anti-rabbit 15 nm gold-conjugated secondary antiserum as a negative control.
Particles of label were counted from three 1 μm2 areas from three area of the cell (pyrenoid and starch sheath, chloroplast excluding pyrenoid and starch sheath, outside of chloroplast) from three cells, for each antibody (APR and SiR) and negative control (secondary antibody only).
A negative control contained secondary antibodies only.
A negative control with secondary antibody was used.
