Sentence examples for secondary mouse from inspiring English sources

Secondary mouse or rabbit HRP-conjugated antibodies (1 5000 or 1 10,000, Cell Signaling, #7074, #7076).

Specimens were then incubated with the secondary mouse anti-human IgG (Fc specific) antibody (1 200) (Jackson Immunoresearch Laboratories, West Grove, PA, USA).

Tumor specimen slides were incubated with the secondary mouse anti-human IgG (Fc specific) antibody (1 200) (Jackson Immunoresearch Laboratories) followed by the tertiary HRP-conjugated goat anti-mouse IgG (Fc specific) antibody (1 400) (DAKO).

To isolate CD44+ faction, there were used primary unlabeled monoclonal antibodies for marker CD44 (BD, 558739) and secondary Mouse IgG1 Magnetic Particles-DM (BD, 557983) according to manufacturer's protocol.

Membranes were washed three times with 5%% milk TTBS, incubated for 60 min with secondary mouse antibodies conjugated to peroxidase (1 3000; Thermo Scientific, Il, USA), and washed again with TTBS to reveal reactivity with the enhanced chemiluminescence (ECL) reagent (Denville Scientific, NJ, USA).

Secondary mouse anti-rabbit antibodies were obtained from Jackson Laboratories.

For CYP2C, a secondary mouse-anti-sheep antibody (213-032-177, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) was used and diluted 1 1000 as described above.

After hI-con1 staining, cells were washed twice with the same buffer and secondary mouse-anti-human antibody (IgG1-FITC, catalog # F0767 Sigma Aldrich, S. Louis, MO) was added for a further 30 minutes.

In an additional experiment, 2 × 10 CD20high blasts engrafted secondary mice and 2 × 10 cellsigh cells purified from the secondary mice engrafted tertiary mice.

One thousand unsorted blasts from the primary mice re-initiated the leukaemia in secondary mice.

The bone-isolated CSCs-like were CD44−CD24+ and showed tumorigenic abilities after injection in secondary mice.