Sentence examples for secondary library from inspiring English sources

The remaining data sets offer enough information to successfully advance three to five scaffolds into the secondary library design.

A secondary library of fragments is designed using the base structures with electron density envelopes from the successful fragment hits of the primary library.

The secondary library compounds are also screened in shape-similar groupings of five that are chosen without the knowledge of binding affinity.

By mutating the sequence of this ligand, a "secondary" library was generated, whose panning on HER2-positive cells isolated a phage-borne peptide with increased specific binding to HER2 (phage NL1.1).

Two advantages of this modification are that it bypasses the lengthy steps of restriction enzyme digestion and ligation, and that the pool of phage clones, recovered after affinity selection, can be used directly to generate a secondary library.

The following religation yielded expression-ready shRNA-vectors (Fig. 1e, secondary library).

Secondary libraries were produced and analyzed as for in vitro selections.

The insert was cloned back into the library backbone plasmid pMT202-6 and the diversity of transformed library plasmids was at least 1×105 clones for such secondary libraries in this and subsequent selections (data not shown).

Normally, 3 4 rounds of panning are done for secondary libraries.

It requires the installation of secondary libraries as well as a C compiler.

Secondary libraries for subtitutional analyses were generated by systematically permutating identified peptides and analyzed by the described peptide spot methodology.