Sentence examples for secondary fixed from inspiring English sources

Open image in new window Fig. 1 A case of 10-year-old boy with post traumatic soft tissue loss managed by multiple surgeries with secondary fixed equines due to soft tissue scarring.

The primary antibody was labeled using an anti-ms IgG Alexa488 secondary, fixed and prepared for imaging by washing in PBS, aspirated and then washed, then adding a 1 ng/ml DAPI/CellMask solution.

Cells/Transwells were rinsed in ice cold distilled water for 3 times, secondary fixed with 1% osmium tetroxide/1.5% potassium ferrocyanide for 2 hours on ice, rinsed 4 times in ice cold distilled water, en bloc stained with freshly prepared and filtered 2% uranyl acetate in distilled water on ice for 2 hours, and rinsed 4 times in ice cold distilled water.

To compare Qdots secondary reagents with organic dye-labeled secondaries, fixed and permeabilized NIH/3T3 cells were stained with mouse α-tubulin for 4 hr at RT, followed by Qdot 655 goat anti-mouse IgG or Alexa 555 goat anti-mouse IgG1 for 1 hr at RT (Fig. 2).

After a secondary fix for 2 h in 1 % osmium tetroxide at 4 °C, the specimens were washed three times in PBS.

In particular, the number of constituent distributions (predominant, residual, primary, secondary) is fixed a priori.

The results of the proximate analysis of the samples, including, the content of primary and secondary volatiles, fixed (solid) carbon and ash (that is, inorganic components of the samples) are summarized in Additional file 6.

The syndrome criteria of 'qi deficiency and blood stasis' and 'qi stagnation and blood stasis' are based on the basic components of the symptom combination; each combination comprises primary symptoms and secondary symptoms (primary symptoms are fixed, secondary symptoms are decided by the patients themselves).

"As far as Japan is concerned, everything is secondary to fixing the economy," says Ed Rogers of Tokyo-based Rogers Investment Advisors.

Opponents of reform say the lack of detail today suggests the government is in retreat, giving itself room to water down proposals for a largely elected second chamber, proposals that many Tories oppose and say are secondary to fixing the economy.

For immunolabeling of the leaf tissue, samples were fixed as described above, but the secondary fixative step was omitted.