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To ensure focus on the early part of the response and not the downstream secondary effects, we undertook a temporal dissection of the response to NB-598 in vitro.
Therefore, in order to exclude any confounding secondary effects, we purposely used spleen-derived EPC from heterozygote mice, which showed normal hematopoietic stem cell numbers.
To investigate whether the diminished insulin signaling downstream of the DAF-2 receptor was due to defective DAF-28 secretion or to other secondary effects, we over expressed Pdaf-28::daf-28 on an extrachromosomal array in animals expressing daf-16::gfp.
To search for secondary effects, we removed the edges in the primary pathway, and repeated the pathway reconstruction procedure.
Considering miR-143 down-regulated genes in our study, including both direct and potential secondary effects, we found an enrichment of genes involved in cell cycle regulation as well as cellular metabolism.
To avoid bias due to secondary effects we identified individuals not yet aware of their condition to explore the association of self-reported lack of sleep and low vitality in relation to IGT in a representative Swedish population.
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2 If we have reason to view one effect (which I will call the primary effect) as more fundamental or more important than the other (the secondary effect), we could use a hierarchical approach.
For the primary effect we use the variance explained by the effect on its own as in the unique approach, and for the secondary effect we use the additional variance explained over and above what was explained by the primary effect.
But simply lying about a medal, creepy though it is, does not have such secondary effects, and we shouldn't want the government in the business of policing false statements made in every context.
To be able to identify the direct effects and separate them from the secondary immunological effects, we examined changes in γδ-T cells at early points during anti-TNF-α therapy.
For simplicity, we ignore here this secondary effect, and we will consider the retarder simpler approximation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com