The photogrammetry method performed very well to track the lateral and vertical displacements at the points on the grid surface as well as the deformed shape of the hinging regions, but the results of secondary calculations, such as rotations of the column, had limited success.
Secondary calculations were made on the connection between subsequent pseudopodia, and include time period between pseudopodia, angle between present and previous pseudopod(s), and distance between start of present pseudopod and end of previous pseudopod.
These secondary calculations may not exclude the influence of some bias factors on the results.
For the secondary calculations of age estimates, an extra significant digit has been reported to allow distinguishing of estimates.
Second, due to the lack of data provided by each study on the association of BMI categories and RA risk, secondary calculations were required in some cases.
PCR results were analyzed with the Roche Lightcycler 480 where the PCR cross point (Cp) value for each amplification curve was determined by a secondary derivative calculation.
It is well known that the structure prediction software, such as RNAfold and Mfold, executes the pre-mRNA secondary structure calculation under simplified conditions, probably resulting in inappropriate minimum free energy predictions.
BL and CT set up and supervised this study, AM and NTM conducted PCR experiments and sequencing, AM performed secondary structure calculation, phylogenetic analysis and estimation of substitution rates of sequence data.
As the secondary structure calculations catch up to the calculated leading edge points, the leading edge has a chance to choose between recently formed secondary structure and a pseudoknot over the same length of sequence.
A discussion of the time complexity of the secondary structure calculations and a theoretical explanation for the contribution from pseudoknots is found in Text S2 (Section S2.3.1).
