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Some of these targets also bind to secondary binding sites of the receptor.
In addition, the novel phosphorylation-independent secondary binding site colored in red is found in the complex structure.
By contrast, the nucleoplasmic LAP2alpha competes with LAP2beta for GLI1 while scaffolding HDAC1 to deacetylate the secondary binding site.
The existence of a lower affinity, secondary binding site (S2) has, however, been debated.
Numerous secondary binding sites for drugs distributed across the protein have also been identified.
A series of potent β2-adrenoceptor agonists incorporating a biarylamine secondary binding group was identified.
More recently, inhibitor design has considered secondary binding sites (exosites) to improve specificity.
Molecular modeling revealed that D3R selectivity depends on the divergence of secondary binding pocket (SBP) in D3R and D2R.
From the biological standpoint, the first application may identify secondary binding sites of drugs that may lead to side-effects.
Additional analyses were used to describe secondary binding sites to electronegative groups such as oxygen or sulfur atom.
A more hydrophobic set of secondary binding groups was explored, prepared rapidly from a common intermediate by Buchwald–Hartwig amination.
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