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Membranes underwent three 5 min TBST washes before a 1 hour, room temperature incubation with secondary antibody (i.e., goat anti-mouse or goat anti-rabbit horseradish peroxidase secondary) at a 1 10,000 dilution in TBST containing 5% BSA.
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Peroxidase-conjugated anti-mouse IgG (Jackson ImmunoResearch) secondary antibodies at a dilution of 1∶20000 were used against 8WG16, whereas peroxidase-conjugated anti-mouse IgM (Sigma) secondary antibodies at a dilution of 1∶5000 were used against both H14 and H5.
At the foot of the tub, I've created a secondary wall at a higher level that allows for an overflow, like a pool with a disappearing edge.
And in many instances, having the right location, even at way above market rent, is preferable to having a secondary location at a lower rent, where the competitor has a primary location.
Mr. Moshayedi sold his shares in a secondary offering – at a nearly 10 percent discount to the market price – when STEC announced its quarterly earnings in August 2009.
A BP spokesman, Robert Wine, denied an Reuters report that said TNK-BP Ltd., the holding company registered in the British Virgin Island that owns TNK-BP International, wouldiscussss a secondary offering at a Sept. 25 board meeting.
These refurbished items are sold to a secondary market at a reduced price.
Figure 2 CDF of the interference due to the secondary network at a primary RX.
Arseniosiderite has been identified as a secondary phase at a French industrial site in which arsenopyrite was stockpiled [50].
The focus is on how to accurately predict typical separating, reattaching and secondary motion at a reasonable computational expense.
The general characteristics of secondary atomization at a range of Weber numbers are also captured in a series of simulations.
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CEO of Professional Science Editing for Scientists @ prosciediting.com