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Kidneys were fixed in a 2.0% paraformaldehyde/2.5% glutaraldehyde phosphate buffered fixative, secondarily fixed with 1% osmium tetroxide, dehydrated using a graded acetone series and infiltrated using Polybed 812 epoxy resin.
Specimens were secondarily fixed by immersion in 1% osmium tetroxide in 0.1 M sodium cacodylate buffer for 2 h and then washed in 0.1 M cacodylate buffer three times, 15 min for each.
The resultant secondarily fixed tissue pieces were rinsed twice in distilled water, dehydrated in graded ethanol, rinsed twice in 100% acetone, placed in a 1∶1 mixture of Spurr's resin and acetone for 30 min, and then transferred to 100% Spurr's resin for 1 h.
The tissues were secondarily fixed in 1% osmium tetroxide buffered in 0.1 M sodium phosphate buffer for 2 h.
Cells were then secondarily fixed with 1% osmium tetroxide and incubated with 1% tannic acid to enhance contrast.
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Numerical analysis of these equations for subsonic speeds (M < 0·3) predicts flutter instability that depends primarily on Λ and the geometry of the fluid enclosure, secondarily on ϵ, and does not depend on M. Experimental results for a relatively fixed geometry, however, show that the onset of instability is completely described by Λ and M, even though Ml 1.
The non-sonicated and sonicated algal cells were primarily-fixed using 4% glutaraldehyde for 2 h, secondarily-fixed using 1% OsO4 for 1 h, and rinsed with 0.1 M cacodylate buffer (pH 7.4).
In every dataset, we fix the number of primarily and secondarily differentially regulated genes to 50 and 172 respectively.
Carbonatitic liquids, enriched in Ca, Si, volatiles, and secondarily Al, migrate in the mantle wedge, react with olivine, and produce clinopyroxene and magnesite, therefore fixing CO2 back again temporarily in the mineral assemblage.
"We keep fixing, fixing, fixing".
Rachel fixes what needs fixing.
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