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The performance criterion (of 80% correct choices) was reached by the second trial block on Day 1 and was sustained through the second trial block on Day 3.
The extinction effect was significantly poorer than that seen in controls however, as indicated by the data obtained in the second trial block on Day 5 (Fig. 2) (P < 0.001).
When we compared the performance of the vehicle-treated animals and the MPEP-treated animals during extinction learning (Day 4, first and second trial blocks) a significant difference was also evident [ANOVA: F 1, 26) = 11.843; P = 0.002], whereby extinction in the second trial block was poorer in MPEP-treated animals compared to controls (P < 0.05).
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The performance on Day 5 was different when the control and MPEP animals were compared [ANOVA: F 1, 26) = 20.495; P < 0.001, first and second trial blocks].
ANOVA of performance across the trial blocks on Day 4 also revealed no significant difference in vehicle and MPEP-treated animals [ANOVA: F 1,26) = 0.818; P = 0.375, first and second trial blocks].
Post-hoc analysis indicated a significant difference in the first trial block (that is the first ten trials collapsed over 21 days) (P < 0.001).
In contrast to the untreated group, the MPEP group showed a renewal effect during the first trial block on Day 5 (Fig. 2) (P < 0.01).
In contrast to the improved task adaptation shown in the present study, reflected by less omissions in the first trial block, galanin transgenic mice exhibited more omissions in the last two trial blocks, pointing to deficiency of sustained attention [ 19].
Post hoc LSD comparisons revealed significant differences between first and second administrations only during the first trial block, P = 0.004 (see Fig. 5), that is, significant interference during the early acquisition of the second administration.
Post hoc analysis revealed that EPO-treated mice had significantly fewer omissions in the first trial block compared with controls (P < 0.05), pointing to a faster adaptation to the task.
This pattern was seen only during the beginning and end of each daily session (contrasts for slopes across cue duration, p = 0.002 and 0.037 for first and third trial blocks, respectively).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com