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Next we compared the original SWC file (representing the first time point) and the edited SWC file (representing the second time point with all structural annotations).
In one subject, a genetic bottleneck was observed, with extensive diversity at the second time point narrowing to two dominant escape forms by the third time point, all within two months of infection.
This was selected as our second time point.
TNF-α was treated at the second time point by a gentle injection through a tubing.
We did not, however, investigate whether these epitopes escaped by sequencing the virus at a second time point.
At the 15 second time point genotype groups did not differ significantly, but exhibited a trend [U = 172.0, z = −1.84, p = 0.07].
To quantify cell spreading, polygons, tracing the cell perimeter were manually marked every second time point, and the projected cell area was calculated.
The second time point was p4, when myelination had already started in control mice and was already evidently impaired in Dicerfl/fl Dhh-Cre+ mice.
The second time point also consisted of one survey.
The second time point was about 24 months after study enrollment.
However, three participants withdrew before the second time point for data collection scheduled at week 4.
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CEO of Professional Science Editing for Scientists @ prosciediting.com