Sentence examples for second thymidine block from inspiring English sources

For inhibitor experiments, the drugs were added for 90 minutes 7.5 hours after release from the second thymidine block.

HeLa cells were collected at different time intervals after the second thymidine block by trypsinizing the cells off the culture dish and fixing them in ice-cold ethanol.

In some experiments, the GSK3 inhibitor SB-216763 was added to the cells 7.5 hours following the second thymidine block for 90 minutes.

To examine chromosomal alignment, we fixed and stained the cells with Hoechst 33342 to visualize DNA 9 hours following the second thymidine block when the majority of cells were in metaphase.

At 0, 2, 5, 6, 9, 12, 16 and 24 hours after release from the second thymidine block, cell-cycle phase distribution was analyzed by flow cytometry with propidium iodide (PI) staining to verify the synchrony.

At 12 h after release from the second thymidine block, cells were harvested for further analysis.

60 pM of GAPDH control siRNAs or SMC2 specific siRNAs were transfected using Oligofectamine (Invitrogen) during S phase, 4 hours after release from the first thymidine block.

Eight hours post transfection, cells were treated with thymidine (4 mM) and 18 h later cells were released from the first thymidine block and either transfected with nontargeting or specific siRNAs.

Pro-metaphase-synchronized cell populations were obtained in the following way: cells were treated with 4 mM thymidine for 18 h, released from the first thymidine block into fresh medium for 6 h and then treated again with thymidine for other 18 h.

Cells were synchronized by a double thymidine-block, and fl-hCdc14B expression was induced by addition of doxycyclin 8 h before cells were released from the second thymidine-block.

A second 2.5 mM thymidine block was introduced for another 16 h.