In a second set of experiments, midbrain sections were double stained with antibodies against phospho-Ser 129 α-synuclein and unmodified α-synuclein.
To evaluate the performance of QUATTRO during data transmission, we reproduced the second set of simulations in Section 5.1, in which an increasing number of nodes are randomly deployed in a fixed-size area.
Meanwhile, the robustness of all three approaches under mild and gross model misspecification is assessed in a second set of simulations in Section 3.2.
The second set of experiments in this section involved flooding core samples with UER water at 5,000 ppm containing calcium concentrations of 332, 664, 1,328, and 1,992 ppm, respectively.
This meant a second set of analysis was conducted (see section on comparison of areas for quality improvement).
A second set of calculations was conducted on 6 sections per spinal cord through 750 µm of tissue spanning the injury center.
The first set of issues is addressed in sections 1 3 of the present article, while sections 4 5 cover the second set of issues.
Nissl substance was stained in the first set of serial sections with 0.5% cresyl violet and myelin was stained according to the method of Gallyas (1979) in another set, whereas two other non-contiguous sets were processed for AChE histochemistry using the protocol described in Kamke et al.
In the second set of experiments, a 0.5 × 0.5 cm section of porcine skin was excised from porcine ear and placed in a saline bath to prevent dehydration and to preserve natural physiological conditions; 27 samples with thicknesses varying from 0.29 mm to 0.91 mm were used.
Sections were then incubated with the second set of primary and secondary antibodies diluted in M.O.M. Diluent for 1 hour.
