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However, the WT mice had an increased DNCB-specific serum IgG level after the second sensitization, which occurred after D7 and reached plateau around D22 (Figure 4C, 4D).
Also, after the second sensitization with DNCB on their shaved back skin, there was a more pronounced skin dermatitis appearing on the backs of the WT mice than on the backs of the B-KO mice (Figure 4E).
These MLNs were collected 24 h after the second sensitization.
Twenty-four hours after the second sensitization, animals in group 2 were sacrificed.
This method of transcriptome profiling appeared to be very sensitive that identified differential expression of hundreds of genes in BALB/c mice within 24 h of the second sensitization with the given food allergens.
In a second sensitization approach, we targeted the antiapoptotic protein XIAP (X-linked inhibitor of apoptosis protein) by RNA interference (RNAi) in TRAIL-resistant pancreatic carcinoma cells and could show that MSC.sTRAILDR4 exerted significantly increased apoptosis-inducing activity as compared with MSC.sTRAIL or MSC.sTRAILDR5.
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This might be because the decorated CdS nanoparticles from the first sensitization could act as the seeds for the deposition of more CdS, which reduced the formation of CdS nanoparticles from bulk reaction and led to the more homogeneous deposition of CdS nanoparticles.
The skin or draining lymph node (DLN) samples were obtained 8 days after the third sensitization course.
At 2 and 4 weeks after the first sensitization, mice were boosted with 100 μg of cBLG emulsified with 0.1 mL of incomplete Freund's adjuvant (Difco Laboratories).
Skin specimens were obtained from patched areas 8 days after the third sensitization course and fixed in 10% buffered formalin and embedded in paraffin.
The mice (n = 10 per group) were fed a diet containing 0%, 0.05%, or 0.5% of heat-killed Lctbs brevis from 2 weeks before the first sensitization to the end of the study.
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