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Chewing gum significantly decreased production time for the second saliva samples by 3 6 min, and had very large effects on assay results, leading to lower IgA and higher T and E in men and women.
After completing the DMS task, participants rated a second time how pleasant/unpleasant and how calm/aroused they felt at the time and gave a second saliva sample.
To evaluate discordance, 10% of the samples were genotyped in duplicate, as was the second saliva DNA sample collected for 18 participants.
Out of the 14 second saliva samples received from individuals recontacted because of poor genotyping completion rates, 12 had improved genotyping rates and 2 did not.
The second saliva sample was taken between the letter and shape experiments and the last saliva sample was taken at the end of the experiment.
The second saliva sample was taken immediately after the particular intervention (learning while treadmill walking or sitting in a canvas chair) was finished.
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Subjects did not drink caffeine for at least 6 hours before the first saliva sample.
Non-steriodal anti-inflammatory drugs were not permitted for at least 72 hours before the first saliva sample as these drugs have been shown to suppress melatonin [25].
However, in our study subjects did not drink alcohol for at least 21.5 hours prior to their saliva collection and were breathalyzed just prior to their first saliva sample to ensure they had zero blood alcohol concentration.
Subjects did not consume any alcohol in at least the 21.5 hours prior to the collection of the first saliva sample and were breathalyzed on arrival to ensure their blood alcohol concentration was zero.
Participants gave their first saliva sample after the training trial.
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