Sentence examples for second round of selection from inspiring English sources

For the second round of selection, the cells selected at the first round were incubated, and β-glycosidases in cells were expressed.

For the transcripts selected in the first step, a second round of selection was performed to include only the most potent predictor genes to allow for assessment of the biological relevance of gene expression data in terms of the GARD assay.

Indeed, negative studies have been published and were included in our initial list of selected papers [ 30, 32, 41] and in the second round of selection [ 20, 23, 26- 29].

In this study the single isolate identified with resistance to RIF only, was subjected to a second round of selection with RFB; after which rpoB gene fragments of 26 randomly selected colonies were re-sequenced in order to identify any additional mutations.

The pools were denatured at 95°C, snap cooled and used to perform the second round of selection using the same procedure as described for first selection.

PolyA-RNA was selected and amplified with Ambion's Aminoallyl MessageAmp II aRNA Kit (Ambion Cat# AM1753) to make antisense-RNA, which was tailed and used for a second round of selection and amplification with the kit to make mRNA.

In addition to the bait selection strategy described above (referred to as one-round selection), we simulated the network coverage results by applying a second round of selections.

The second round of selections differed by reduction of the amount of ligand to 5 μl Dynabeads coated with 0.01 μg xyloglucan.

Access to these requirements can be provided in case the general selection scheme (illustrated in Fig.  1) is streamlined in that only the second round of selections (step 3) is stringently controlled whereas step 2 obeys relaxed conditions that can be verified with just moderate investments of time.

Thus, in the first and second rounds of selection, the hydrolytic activity was measured using pNP-β-d-glucopyranoside as a substrate.

Images of calli growth in the whole dish after the first and second rounds of selection were taken using a SONY α550 digital camera.