If QC is passed, the primary hits are selected for a second round of screening.
After the second round of screening, 20 compounds with an inhibition rate higher than 20% were selected as inhibitors of the interaction between Tob and CNOT7 (Fig. 1G).
A second round of screening was expected to reduce the list to about 950 names, but no such list of finalists was ever issued.
Then it's separated into bottles which are kept in a -80C fridge for months, where they wait for donors to pass a second round of screening.
Peptides with the potential avidity were further verified by a second round of screening.
Positive clones were confirmed in a second round of screening in yeast and sequenced.
After this second round of screening, 14 mutants remained that reproducibly displayed an increased frequency of ascal GFP fluorescence.
Pools of 16 or fewer peptides were utilized for a first round of screening, followed by 4×4 matrices to identify individual peptide candidates for a second round of screening, followed by confirmation of individual peptides in a third round of screening.
The most active pool was selected for the second round of screening, subdivided into pools of 5 peptides and then into individual peptides.
Twenty of the pools identified in the first round of screening were selected for a second round of screening in order to isolate the corresponding p53/Mdm2 modifier.
