Sentence examples for second round of database from inspiring English sources

Related family members were identified by a second round of database searching against GenBank to ensure all available genomic data was utilized.

To use all available sequence data, CTG proteins with a top database hit to a bacterial protein in UniProt were extracted for a second round of database searching against GenBank (E value of 10-20).

Following a first round of database searching and on-target recrystallization of the sample spots, MS/MS spectra were collected on selected precursors (see Additional file 3 for detailed information).

Peptide mass fingerprint (PMF) data were collected on an UltraflexIII MALDI ToF/ToF mass spectrometer (Bruker Daltonics, Bremen, Germany) and used for a first round of database searching using MASCOT 2.3 (http://www.matrixscience.com) against NCBI nr core collection and the potato genome sequencing consortium (PGSC) Solanum tuberosum group Phureja DM protein database.

The consensus sequences from all validated contigs were used as query sequences in a second round of TBLASTN analysis of the Pinophyta database subset to identify sequences that were not initially identified in the original TBLASTN searches, both to extend the contigs and to ensure that no CLE or CLEL sequences were overlooked due to truncated sequences lacking a CLE or CLEL domain.

This second round of clustering resulted in a Sanger-based database of 12,329 unigenes from Alexandrium.

In a second round of BLASTX comparisons against the nr-NCBI database, 28.5% of the contigs and 9.2% of the singlets were classified as putative orthologs.

After this round of mapping, 13,073,825 unmapped reads were obtained that were subsequently aligned against the 5′ UTR (1000 bp) and 3′ UTR (1000 bp) databases for the second round of mapping, after which 6,995,901 unmapped reads were obtained.

Those contigs that lacked matches were then subjected to a second round of BLASTX search against the larger NCBI non-redundant database.

To this end, N/C out-of-core ends having length greater than 100 are stored in the database and they are used to launch the second round of the procedure at a single node of the evolutionary tree (see the "Second round" section below).

The second round of BLAST searches were performed against the SwissProt, RefSeq and nr databases via the platform Blast2GO (Blast2GO