Suggestions(5)
Exact(9)
A second round of analyses was still performed, in which only those samples including 20 individuals at least were selected.
A second round of analyses using an independent series of controls (CG2) could not, however, confirm the initial finding, and instead suggested another mtSNP candidate with a marginal significant P-value, namely A4769G.
In the second round of analyses homozygotes for the minor allele were grouped together with heterozygotes.
This second round of analyses was performed in different rooms and by different individuals.
In the second round of analyses, this fact was explained by the presence of intratumoural heterogeneity.
The results were independently scored by two observers and a second round of analyses confirmed the results.
Similar(51)
The simplicity of this approach makes it an appealing choice for conducting a first round of analyses.
Thus, at the end of the first round of analyses, the results from the two techniques in a total of eight cases were discordant.
Results that were statistically significant in the first round of analyses but non-significant when excluding the neocortex volumes (or vice versa) have to be judged carefully (Additional files 5 and 6).
Then, a second round of phylogenetic analyses was performed on these orthologues.
To more specifically examine how AvTD and VarTD were related to latitude at particular depths (and vice versa), and to more fully investigate significant depth x latitude interactions (see Results), we conducted a second round of GAM (identity link, Gaussian distribution) analyses in which we binned data either by depth or by latitude zones.
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