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In contrast, p185BCR/ABL-positive cells did not exhibit colonies in the second plating round, but many viable cells were present that were apparently unable to give rise to colonies (Figure 5D).
The second plating contained a majority of epithelial cells and a considerable number of connective tissue cells.
The second plating contained predominantly epithelial cells and a few connective tissue cells but only a small percentage of the cells remained viable after 24 h in the supernatant.
If no characteristic pathogenic Y. enterocolitica colonies were visible on the CIN agar plate following KOH treatment after 24 h incubation of enrichment, a second plating on CIN agar following KOH treatment was performed after 48 h of enrichment.
Colonies that appeared within 3 days after the second plating event were believed to be true positives and 5 representatives from each construct and zeocin level were further analysed.
Enquiry into the difference between the first 56 and the later 106 samples with the sample source sites revealed that the first 56 were plated in two independent events at laboratory C, with laboratory C and laboratory A receiving DNA from the first plating event and laboratory B from the second plating event.
Similar(53)
Bone marrow cells were first plated onto tissue culture plastic (30 min, 37°C) and the adherent (macrophage-rich) cells discarded.
For all experiments, HepG2 cells were first plated on to collagen-coated plates.
To obtain D. discoideum fruiting bodies, we first plated 10 D.
Briefly, 1 ml culture medium with 0.4% agar was first plated into each well of a 12-well plate.
Invert second plate over plate with fish.
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