That makes it the second largest domain purchase this year, following Toys R Us' acquisition of toys.com.
Domain registrations for.org's have risen recently, up 11% in the first half of 2009 over the same period a year before according to pir.org Currently there are over 7 million.org domain registrations, making.org's the third largest domain extension, trailing only.com and.net.net
At $4.9 million, the Clothes.com sale would have been the second largest reported domain-name transaction in 2008 after Fund.com ($10 million), and the second biggest one so far this year after Toys.com ($5.1 million).
These proteins possess a single transmembrane spanner (TMS) at their N-terminus followed by a large extracytoplasmic domain, six additional TMSs, a second large extracytoplasmic domain, and five final C-terminal TMSs.
The three methionines of CusA described to be important for copper resistance are located in the second large periplasmic domain [5].
CusA and AcrB sequence alignment indicated that these fragments probably resulted from a cleavage in the beginning of the second large periplasmic domain of CusA (fig. 5) and thus corresponded to 7 TM helices.
The second largest group consisted of AP2-domain transcription factors (8), including two DRE binding proteins DREB1B (CBF1, At4g25490) and DREB2A (Athat5410) thavehave been well characterized in regulation of cold responsive and dehydration responsive genes, respectively [ 7, 41].
In addition to the classical ParB toxin domains, we recovered a second large group of toxin domains typified by that found in Neisseria gonorrhoeae NGK_2271 (gi: 194099761), which could be united using profile-profile comparisons with the ParB domain (HHpred probability 93%; p = 2x10-6 matoh to 1vz0 Thermus ParB).
Myosin II is the second largest class of myosins, and is characterized by a myosin N-terminal domain (PF02736) and a tail containing an IQ domain and a myosin tail domain (PF01576), consisting of several coiled-coil domains.
The phosphotyrosine-binding (PTB) or phosphotyrosine-interacting domain, first identified in the adaptor protein Shc [ 97- 99], is the second largest family of pTyr-binding modules.
