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Three additional sequences (Clo4-B, Clo10-2-D, Clo11-A) were obtained from the WSS derived from second generation sequences from genomic DNA.
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Ganal et al. [ 12] reviewed recent SNP identification methods including DNA arrays, amplicon sequencing, mining existing EST resources, and using sequence data generated with second generation sequencing technologies.
Illumina second generation sequencing, which is suitable for generating enormous numbers of transcript sequences that can be used for functional genomic analysis of longan.
Second generation sequencing.
Genome-wide studies of bacterial gene expression are shifting from microarray technology to second generation sequencing platforms.
We employed hybridization capture methods and second generation sequencing technology to obtain >30 kb of exon sequences from 26 nuclear genes for both H. gigas and Dugong dugon.
Recent studies have demonstrated the capability of second generation sequencing (SGS) to provide coverage of short tandem repeats (STRs) found within the human genome.
This again demonstrates the applicability and scalability of second generation sequencing techniques for the detection of mutations in FFPE tissues.
Second generation sequencing has prompted a number of groups to re-interrogate the transcriptomes of several bacterial and archaeal species.
With the power of second generation sequencing techniques, both sets of information will be achieved in one process.
New deep sequencing technologies (also called next-generation or second generation sequencing methods) are now available [1] [3] to study the transcriptome in unprecedented detail (RNA-Seq, [4]).
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