"second extraction" is correct and usable in written English.
You can use this term when referring to the second of two extractions, or when you are referring to the process of retrieving something for the second time. For example, "After the first extraction was unsuccessful, we tried a second extraction to gather more detailed data."
Following the second extraction, the two extracts were pooled and kept frozen at -20°C until analysis as described by Glassop et al. [ 57].
It showed that most THSG was extracted (> 99%) after the second extraction.
After digestion, the peptides were first extracted with acetonitrile/ammonium bicarbonate, followed by a second extraction with formic acid.
The solid residue of the second extraction step was discarded and the combined extract was centrifuged again for 5 min (3,500 rpm).
However, the proportion of 18 3 in the extract decreased from the first to the second extraction and the ratio of saturated to unsaturated fatty acid increased from the first to the second extraction.
We performed a second extraction by adding chloroform:isoamyl alcohol (24:1), mixing, and extracting the aqueous layer.
A drawback of this procedure is that more matrix compounds are extracted because of the high water content of the second extraction solvent.
To determine the efficiency of intact biopsy extraction, we first extracted metabolites from intact biopsy samples and then ground the tissue and performed a second extraction.
Decant the liquid from the second extraction into the same 20 mL volumetric flask.
Meanwhile, an CIP extraction efficiency of 85.6 % is achieved in the second extraction.
For fennel, savoury and cotton lavender, the external mass transfer and the equilibrium phase also influenced the second extraction period, since ks changed with the tested flow rates.
