The media were changed after 5 days, and thereafter every second day for 21 days.
A-3656, Sigma, Stockholm, Sweden) every second day for 4 days reaching the equivalent to PN11 (short term: PN5+2 days in vitro+4 days in vitro with treatment) or to PN19 (long term: PN5+2 days in vitro +12 days in vitro with treatment).
We will play every second day for 10 days.
Cells were collected every second day for 14 days.
Nitroglycerin was administered IP to mice every second day for 9 days.
Treated A. ferus eggs were subsequently left on moist filter paper in a ventilated Petri dish and observed every second day for 40 days to assess viability.
Treated H. ligniperda eggs were monitored every second day for 32 days, and successful hatch was deemed indicative of non-sterility.
Serum-free keratinocyte-defined medium was changed every second day for 6 days.
The maximum tolerated dose (MTD) of dexrazoxane in mice is 250 mg kg−1, 7 times higher than our dose of 35 mg kg−1, which was given every second day for 17 days.
We transnasally delivered a bolus of 50 µl bleomycin (0.05 U/mouse; Blenoxane, Bristol-Myers Squib, New York, USA), or 2 µg HYPER-IL-6 every second day for 14 days to anaesthetized mice.
Blood was drawn to measure cortisol, corticotropin (ACTH), dehydroepiandrosterone-sulphate (DHEAS), IL-6 and tumor necrosis factor alpha (TNF-α) levels within 48 hours of the onset of sepsis and/or septic shock, and thereafter every second day for 14 days, or until death or ICU discharge.
