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The phrase 'second course of chemotherapy' is correct and usable in written English.
You can use it to refer to additional rounds of chemotherapy that a patient has undergone after the first course. For example: "After unfortunate results from the first course of chemotherapy, the doctor recommended a second course of chemotherapy."
Exact(20)
I had two weeks off after my second course of chemotherapy in order to breathe the air of the outside world and recharge my batteries and I was due to be readmitted for a stem-cell transplant on 27 May.
She is now seven months into her second course of chemotherapy.
The second course of chemotherapy was performed 2 3 days after the completion of RT.
This second course of chemotherapy led to remission and the same chemotherapy regimen was repeated.
The second course of chemotherapy will commence on calendar day 29 [ 4, 9].
After the second course of chemotherapy, hypertension developed in the patient.
Similar(40)
Similar results were reported by Schelling and colleagues after the first and second courses of chemotherapy in 22 patients [ 76].
Radiotherapy to the primary lesion of 25 Gy in 13 fractions was given after each of the first and second courses of chemotherapy.
The only observed variation was a slight decrease (10%) in IGF-1 concentrations, assayed before treatment, between the first and the second courses of chemotherapy.
Less than a year after Dido's first course of chemotherapy, the tumours were back.
The First Lady hugged Maysie and Presley Cogdell, who were there with their mother Trudi, 44, who is on her third course of chemotherapy to treat the breast cancer which has spread to her bone and liver.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com