Sentence examples for second constructing from inspiring English sources

The procedure involved, first, optimizing the electrotransformation conditions of the Xac 306 strain and, second, constructing non-replicative suicide vectors carrying knockout copies of the target gene.

The second construct requires more explanation.

The second construct of performance anxiety concerns test anxiety.

The Cronbach's alpha was 0.93 for all 33 items, 0.90 for the first construct, and 0.87 for the second construct.

These notions suggest that the second construct of test anxiety applies to Japanese EFL situations.

The second construct contains nucleotides 1 45 of the Eln 3' UTR with the wildtype sequence.

The second construct is based on an E. coli codon optimized gene of Bet v 1.0401.

We microinject the first construct and immediately electroporate it and right after the first electroporation we proceed the same way for the second construct.

Phylogenetic trees were first constructed using the NCBI database and the Blast and tree construction programs.

"But someone in the private sector has to first construct those buildings.

Parts of the wall have been rebuilt during the two millennia since it was first constructed.